Protein Purification Reagents and Kits
KAYAKU ADVANCED MATERIALS INC MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
Takara Bio Shield1, 500 ul
The Shield1 compound stabilizes a protein of interest tagged with the destabilization domain (DD), which is based on a mutated version of the FKBP protein. It is used to protect the DD fusion protein of interest from proteasomal degradation. The amount of Shield1 can be varied to tune the amount of stabilized protein of interest within the cell. 500 ul
STEMCELL Technologies EasySep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.
CUBE BIOTECH purecube 100 compact cartridge ni-indigo 1x1 ml
purecube 100 compact cartridge ni-indigo 1x1 ml
STEMCELL Technologies EasySep™ Human T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Epigentek Inc EpiMag HT (96-Well) Magnetic Separator (1/Pack)
The EpiMag HT (96-Well) Magnetic Separator is a magnetic stand (magnetic rack) that allows paramagnetic bead precipitation of liquid samples from various flat-bottom or U-bottom 96-well microplates (we recommend our EpiMag 96-Well Microplates for ideal compatibility). It can be used for isolation and purification of nucleic acids and proteins, immunoprecipitation, immunoassays (ELISA), cell sorting, and purification of biomolecules. A PCR adapter is also included for the option of using V-bottom semi-skirted/unskirted PCR microplates.
Hampton Research SOLUBILITY & STABILITY SCREEN
HR2-072/Solubility & Stability Screen - 0.5 ml, Deep Well block format. Please note items from this supplier are non-returnable.
Sartorius Biosensor / Ni-NTA (NTA) Tray
Ni-NTA (NTA) Biosensors - One tray of 96 biosensors coated with nickel-charged tris-NTA for both quantitation and kinetic applications of HIS-tagged molecules.
Surmodics Inc StabilGuard Immunoassay Stabilizer, 50mL
StabilGuard™ Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper. The key benefit of the protein free formulation is the elimination of interference and cross reactivity within immunoassay applications.
IBA LifeSciences Strep-TactinXT PE , 50 µl
Strep-TactinXT conjugated to R-Phycoerythrin (PE) is optimized for flow cytometry of cells. It binds to Strep-tagII- or Twin-Strep-tag- fusion proteins with a high affinity.Cells can either be stained directly if they express a strep-tagged surface protein or indirectly via a strep-tagged protein that subsequently binds to the cells (e.g. for sorting antigen-specific B cells). Independent from the chosen method, biotin addition will cause the release of Strep-TactinXT PE from the cell surface. This enables the use of selected cells for further downstream applications.This conjugate is especially recommended for low-affinity or low-expressed targets.
STEMCELL Technologies EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit is designed to isolate naïve CD62L+CD44-CD8+ T cells from single-cell suspensions of splenocytes or lymph nodes by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies that are directed against non-naïve CD8+ T cells (CD4, CD11b, CD11c, CD19, CD44, CD45R/B220, CD49b, TCRγ/δ, TER119) and streptavidin-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Neuromics, Inc BioLock Biotin Blocking Solution, 50 ml
Cultivation media often contain significant amounts of biotin. This is especially the case for mammalian or insect cell culture media.Thus, if proteins from biotin containing extracts or media are intended to be purified via Strep-Tactin chromatography, biotin must be masked by the addition of avidin prior to the application onto the column. BioLock biotin blocking solution allows this masking of biotin (and biotinylated proteins) in a very fast and convenient way.Activity: >70U/mlAdd at least 1U of BioLock solution per µg of biotin.